新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查

doi:10.11843/j.issn.0366-6964.2015.06.017

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 1004-1010.doi: 10.11843/j.issn.0366-6964.2015.06.017

新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查

肖云霞¹，徐新龙²，尹小平²，王安东¹，闫遵祥¹，段成任³，徐军^2*

(1.石河子大学动物科技学院，石河子 832000；2.阿拉山口出入境检验检疫局，阿拉山口 833418；3.新疆农业大学动物医学学院，乌鲁木齐 830052）

收稿日期:2014-10-28 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 徐军，高级兽医师，主要从事动物检疫研究，E-maill：alskxj@sina.com
作者简介:肖云霞（1990-），女，新疆乌鲁木齐人，硕士生，主要从事兽医卫生监督与管理研究，E-mail：18675016094@163.com
基金资助:
国家质检总局科技计划项目（2014IK239）

Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port

XIAO Yun-xia¹，XU Xin-long²，YIN Xiao-ping²，WANG An-dong¹，YAN Zun-xiang¹，DUAN Cheng-ren³，XU Jun^2*

(1.College of Animal Science and Technology，Shihezi University，Shihezi 832000，China；2.Ala-shankou Entry-exit Inspection and Quarantine Bureau of the P.R.China，Alashankou 833418，China；3.College of Veterinary Medicine，Xinjiang Agricultural University，Urumchi 830052，China)

Received:2014-10-28 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

对新疆阿拉山口口岸地区鼠类棘球蚴感染情况进行系统调查。选择阿拉山口口岸三个不同区域（野外区、城郊综合区和城区），2013—2014年连续2年采集鼠类样本；剖检、分离肝，对所有样本提取肝基因组DNA，利用聚合酶链式反应扩增棘球蚴线粒体细胞色素C氧化酶亚单位1（Cox1）、NADH脱氢酶（ND1）等基因并测序。剖检发现8份样品具有包囊，感染率为2.08%；分子鉴定发现有10份样品呈PCR阳性，感染率为2.60%，Cox1基因Blast分析中有8份样品与青海检测的苏俄多房棘球绦虫的相似性最高（登录号：JQ 690286.1），为85%；ND1基因Blast分析，另2份样品与多房棘球蚴相似性最高（登录号：AB 720065.1），为100%；统计学分析发现野外区、城郊综合区鼠类感染率分别为2.74%和2.38%，而城区未检测到病原体。阿拉山口口岸野外区野鼠棘球蚴感染率最高，易感鼠种为大沙鼠。

Abstract:

The infectious status of echinococcosis in wild rodents was investigated at different areas of Alashankou Port.The wild rodent samples were collected from rural，suburb and urban area of Ala-shankou Port during 2013-2014.Then，pathological sections of liver were prepared and stained with H&E；the genomic DNA of livers and cysts were extracted.Cytochrome C oxidase 1 (Cox1)，NADH dehydrogenase subunit 1 (ND1) in Echinococcus mitochondria were amplified from the DNA and sequenced.Results were as follows：eight samples with cyst were found in the process of autopsy，and the positive rate was 2.08%.Ten positive samples were detected positive by PCR，and the positive rate was 2.60%.The analysis of Blast showed that 8 samples of our sequences were clustered with that of Echinococcus sp. (Accession No.JQ 690286.1)，and their identities were 85%.The remaining 2 samples of our sequences were clustered with that of Echinococcus multilocularis (Accession NO：AB 720065.1)，and their identities were 100%.The results of statitical analysis conveyed us the infection rates of echinococcosis at rural and suburb area were 2.74% and 2.38%，respectively，while no positive sample was detected at urban area.The results presented here demonstrate that the wild rodents at rural area of Ala-shankou Port showed the highest infection rate，and its susceptible host was the Rhombomys opimus.

中图分类号:

S853.734

肖云霞，徐新龙，尹小平，王安东，闫遵祥，段成任，徐军. 新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查[J]. 畜牧兽医学报, 2015, 46(6): 1004-1010.

XIAO Yun-xia，XU Xin-long，YIN Xiao-ping，WANG An-dong，YAN Zun-xiang，DUAN Cheng-ren，XU Jun. Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1004-1010.

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新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查

Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port

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